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Cross-Platform Evaluation of Commercial Real-Time SYBR Green RT-PCR Kits for Sensitive and Rapid Detection of European Bat Lyssavirus Type 1

This study evaluates the performance of five two-step SYBR Green RT-qPCR kits and five one-step SYBR Green qRT-PCR kits using real-time PCR assays. Two real-time thermocyclers showing different throughput capacities were used. The analysed performance evaluation criteria included the generation of s...

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Detalles Bibliográficos
Autores principales: Picard-Meyer, Evelyne, Peytavin de Garam, Carine, Schereffer, Jean Luc, Marchal, Clotilde, Robardet, Emmanuelle, Cliquet, Florence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345247/
https://www.ncbi.nlm.nih.gov/pubmed/25785274
http://dx.doi.org/10.1155/2015/839518