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High-throughput preparation methods of crude extract for robust cell-free protein synthesis

Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful technology platform for high-throughput protein production and genetic part characterization. Unfortunately, robust preparation of highly active extracts generally requires specialized and costly equipment and can be la...

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Detalles Bibliográficos
Autores principales: Kwon, Yong-Chan, Jewett, Michael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345344/
https://www.ncbi.nlm.nih.gov/pubmed/25727242
http://dx.doi.org/10.1038/srep08663