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High-throughput preparation methods of crude extract for robust cell-free protein synthesis
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful technology platform for high-throughput protein production and genetic part characterization. Unfortunately, robust preparation of highly active extracts generally requires specialized and costly equipment and can be la...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345344/ https://www.ncbi.nlm.nih.gov/pubmed/25727242 http://dx.doi.org/10.1038/srep08663 |