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Functional Properties of the Catalytic Domain of Mouse Acidic Mammalian Chitinase Expressed in Escherichia coli

Mouse acidic mammalian chitinase (AMCase) plays important physiological roles in defense and nutrition. AMCase is composed of an N-terminal catalytic domain (CatD) and a C-terminal chitin-binding domain (CBD). We expressed CatD of mouse AMCase as a recombinant fusion protein with Protein A and V5-Hi...

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Detalles Bibliográficos
Autores principales: Kashimura, Akinori, Kimura, Masahiro, Okawa, Kazuaki, Suzuki, Hirotaka, Ukita, Atsushi, Wakita, Satoshi, Okazaki, Kana, Ohno, Misa, Bauer, Peter O., Sakaguchi, Masayoshi, Sugahara, Yasusato, Oyama, Fumitaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346942/
https://www.ncbi.nlm.nih.gov/pubmed/25689423
http://dx.doi.org/10.3390/ijms16024028