Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer's disease: Effects and a comparison with donepezil☆

After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer's disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of...

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Autores principales: Qin, Ren’an, Zhou, Desheng, Wang, Jiajun, Hu, Hua, Yang, Yang, Yao, Xiaoxuan, Sun, Xiaopeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Research and Report: Traditional Chinese Medicine and Neuroregeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347004/
https://www.ncbi.nlm.nih.gov/pubmed/25745459
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.003
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author Qin, Ren’an
Zhou, Desheng
Wang, Jiajun
Hu, Hua
Yang, Yang
Yao, Xiaoxuan
Sun, Xiaopeng
author_facet Qin, Ren’an
Zhou, Desheng
Wang, Jiajun
Hu, Hua
Yang, Yang
Yao, Xiaoxuan
Sun, Xiaopeng
author_sort Qin, Ren’an
collection PubMed
description After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer's disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer's disease, with similar effects to donepezil.
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spelling pubmed-43470042015-03-05 Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer's disease: Effects and a comparison with donepezil☆ Qin, Ren’an Zhou, Desheng Wang, Jiajun Hu, Hua Yang, Yang Yao, Xiaoxuan Sun, Xiaopeng Neural Regen Res Research and Report: Traditional Chinese Medicine and Neuroregeneration After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer's disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer's disease, with similar effects to donepezil. Medknow Publications & Media Pvt Ltd 2012-03-25 /pmc/articles/PMC4347004/ /pubmed/25745459 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.003 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report: Traditional Chinese Medicine and Neuroregeneration
Qin, Ren’an
Zhou, Desheng
Wang, Jiajun
Hu, Hua
Yang, Yang
Yao, Xiaoxuan
Sun, Xiaopeng
Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer's disease: Effects and a comparison with donepezil☆
title Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer's disease: Effects and a comparison with donepezil☆
title_full Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer's disease: Effects and a comparison with donepezil☆
title_fullStr Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer's disease: Effects and a comparison with donepezil☆
title_full_unstemmed Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer's disease: Effects and a comparison with donepezil☆
title_short Compound Danshen tablets downregulate amyloid protein precursor mRNA expression in a transgenic cell model of Alzheimer's disease: Effects and a comparison with donepezil☆
title_sort compound danshen tablets downregulate amyloid protein precursor mrna expression in a transgenic cell model of alzheimer's disease: effects and a comparison with donepezil☆
topic Research and Report: Traditional Chinese Medicine and Neuroregeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347004/
https://www.ncbi.nlm.nih.gov/pubmed/25745459
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.003
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