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Challenges with Using Primer IDs to Improve Accuracy of Next Generation Sequencing
Next generation sequencing technologies, like ultra-deep pyrosequencing (UDPS), allows detailed investigation of complex populations, like RNA viruses, but its utility is limited by errors introduced during sample preparation and sequencing. By tagging each individual cDNA molecule with barcodes, re...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351057/ https://www.ncbi.nlm.nih.gov/pubmed/25741706 http://dx.doi.org/10.1371/journal.pone.0119123 |