Cargando…

Challenges with Using Primer IDs to Improve Accuracy of Next Generation Sequencing

Next generation sequencing technologies, like ultra-deep pyrosequencing (UDPS), allows detailed investigation of complex populations, like RNA viruses, but its utility is limited by errors introduced during sample preparation and sequencing. By tagging each individual cDNA molecule with barcodes, re...

Descripción completa

Detalles Bibliográficos
Autores principales:	Brodin, Johanna, Hedskog, Charlotte, Heddini, Alexander, Benard, Emmanuel, Neher, Richard A., Mild, Mattias, Albert, Jan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351057/ https://www.ncbi.nlm.nih.gov/pubmed/25741706 http://dx.doi.org/10.1371/journal.pone.0119123

Ejemplares similares

PCR-Induced Transitions Are the Major Source of Error in Cleaned Ultra-Deep Pyrosequencing Data
por: Brodin, Johanna, et al.
Publicado: (2013)

Performance of Ultra-Deep Pyrosequencing in Analysis of HIV-1 pol Gene Variation
por: Mild, Mattias, et al.
Publicado: (2011)

Dynamics of HIV-1 Quasispecies during Antiviral Treatment Dissected Using Ultra-Deep Pyrosequencing
por: Hedskog, Charlotte, et al.
Publicado: (2010)

Estimating time of HIV-1 infection from next-generation sequence diversity
por: Puller, Vadim, et al.
Publicado: (2017)

MPD: multiplex primer design for next-generation targeted sequencing
por: Wingo, Thomas S., et al.
Publicado: (2017)

The Accuracy, Feasibility and Challenges of Sequencing Short Tandem Repeats Using Next-Generation Sequencing Platforms
por: Zavodna, Monika, et al.
Publicado: (2014)

Methods to improve the accuracy of next-generation sequencing
por: Cheng, Chu, et al.
Publicado: (2023)

Optimus Primer: A PCR enrichment primer design program for next-generation sequencing of human exonic regions
por: Brown, Andrew MK, et al.
Publicado: (2010)

A Primer for Disease Gene Prioritization Using Next-Generation Sequencing Data
por: Wang, Shuoguo, et al.
Publicado: (2013)

Novel Primer Sets for Next Generation Sequencing-Based Analyses of Water Quality
por: Lee, Elvina, et al.
Publicado: (2017)

A Next-Generation Sequencing Primer—How Does It Work and What Can It Do?
por: Alekseyev, Yuriy O., et al.
Publicado: (2018)

Theoretical and experimental assessment of degenerate primer tagging in ultra-deep applications of next-generation sequencing
por: Liang, Richard H., et al.
Publicado: (2014)

An efficient and cost-effective method for primer-induced nucleotide labeling for massive sequencing on next-generation sequencing platforms
por: Guo, Junjie, et al.
Publicado: (2019)

HGT-ID: an efficient and sensitive workflow to detect human-viral insertion sites using next-generation sequencing data
por: Baheti, Saurabh, et al.
Publicado: (2018)

Next Generation Sequencing Technology in the Clinic and Its Challenges
por: Vestergaard, Lau K., et al.
Publicado: (2021)

Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing
por: Takahashi, Shunsuke, et al.
Publicado: (2014)

Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing(1)
por: Celik, Mélina, et al.
Publicado: (2015)

BAMClipper: removing primers from alignments to minimize false-negative mutations in amplicon next-generation sequencing
por: Au, Chun Hang, et al.
Publicado: (2017)

A universal primer-independent next-generation sequencing approach for investigations of norovirus outbreaks and novel variants
por: Fonager, Jannik, et al.
Publicado: (2017)

Next-generation sequencing of newborn screening genes: the accuracy of short-read mapping
por: Trier, C., et al.
Publicado: (2020)

Diagnostic accuracy of metagenomic next-generation sequencing for cryptococcosis in immunocompetent and immunocompromised patients
por: Su, Yi, et al.
Publicado: (2022)

Improved accuracy of etiological diagnosis of spinal infection by metagenomic next-generation sequencing
por: Xu, Liang, et al.
Publicado: (2022)

Methods, Challenges, and Promise of Next-Generation Sequencing in Cancer Biology
por: Haimovich, Adrian D.
Publicado: (2011)

Pharmacogenomics in Children: Advantages and Challenges of Next Generation Sequencing Applications
por: Vanakker, O. M., et al.
Publicado: (2013)

Genomics in 2012: challenges and opportunities in the next generation sequencing era
por: Zhao, Zhongming, et al.
Publicado: (2012)

Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis
por: Kolb, Manon, et al.
Publicado: (2019)

Single primer isothermal amplification (SPIA) combined with next generation sequencing provides complete bovine coronavirus genome coverage and higher sequence depth compared to sequence-independent single primer amplification (SISPA)
por: Myrmel, Mette, et al.
Publicado: (2017)

In vivo mutation rates and the landscape of fitness costs of HIV-1
por: Zanini, Fabio, et al.
Publicado: (2017)

Coval: Improving Alignment Quality and Variant Calling Accuracy for Next-Generation Sequencing Data
por: Kosugi, Shunichi, et al.
Publicado: (2013)

Next‐generation sequencing improves agreement and accuracy in the diagnosis of Spitz and spitzoid melanocytic lesions
por: Roth, Andrew, et al.
Publicado: (2022)

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies
por: Klindworth, Anna, et al.
Publicado: (2013)

An Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens
por: WANG, Chun Hua, et al.
Publicado: (2017)

Comparison of 16S rRNA Gene Based Microbial Profiling Using Five Next-Generation Sequencers and Various Primers
por: Park, Changwoo, et al.
Publicado: (2021)

Next-Generation Sequencing
por: Xiong, Momiao, et al.
Publicado: (2010)

Next-generation sequencing
por: Reis-Filho, Jorge S
Publicado: (2009)

Challenges in the introduction of next-generation sequencing (NGS) for diagnostics of myeloid malignancies into clinical routine use
por: Bacher, Ulrike, et al.
Publicado: (2018)

Next-Generation Sequencing in Clinical Molecular Diagnostics of Cancer: Advantages and Challenges
por: Luthra, Rajyalakshmi, et al.
Publicado: (2015)

Challenges in the Setup of Large-scale Next-Generation Sequencing Analysis Workflows
por: Kulkarni, Pranav, et al.
Publicado: (2017)

Mycobacterium tuberculosis Next-Generation Whole Genome Sequencing: Opportunities and Challenges
por: Iketleng, Thato, et al.
Publicado: (2018)

The role of Next-Generation Sequencing in tumoral radiosensitivity prediction
por: Bibault, Jean-Emmanuel, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_p3l247v8k0d0tc6u93rfa9c5af