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The Effects of Freezing on Faecal Microbiota as Determined Using MiSeq Sequencing and Culture-Based Investigations

BACKGROUND: High-throughput sequencing has enabled detailed insights into complex microbial environments, including the human gut microbiota. The accuracy of the sequencing data however, is reliant upon appropriate storage of the samples prior to DNA extraction. The aim of this study was to conduct...

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Detalles Bibliográficos
Autores principales: Fouhy, Fiona, Deane, Jennifer, Rea, Mary C., O’Sullivan, Órla, Ross, R. Paul, O’Callaghan, Grace, Plant, Barry J., Stanton, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352061/
https://www.ncbi.nlm.nih.gov/pubmed/25748176
http://dx.doi.org/10.1371/journal.pone.0119355