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Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci

Developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. In the present work, a novel chimeric lysin (Ply187N-V12C) was constructed by fusing the catalytic domain (Ply187N) of the bacteriophage lysin Ply187 with the...

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Autores principales: Dong, Qiuhua, Wang, Jing, Yang, Hang, Wei, Cuihua, Yu, Junping, Zhang, Yun, Huang, Yanling, Zhang, Xian-En, Wei, Hongping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353335/
https://www.ncbi.nlm.nih.gov/pubmed/25219798
http://dx.doi.org/10.1111/1751-7915.12166
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author Dong, Qiuhua
Wang, Jing
Yang, Hang
Wei, Cuihua
Yu, Junping
Zhang, Yun
Huang, Yanling
Zhang, Xian-En
Wei, Hongping
author_facet Dong, Qiuhua
Wang, Jing
Yang, Hang
Wei, Cuihua
Yu, Junping
Zhang, Yun
Huang, Yanling
Zhang, Xian-En
Wei, Hongping
author_sort Dong, Qiuhua
collection PubMed
description Developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. In the present work, a novel chimeric lysin (Ply187N-V12C) was constructed by fusing the catalytic domain (Ply187N) of the bacteriophage lysin Ply187 with the cell binding domain (146-314aa, V12C) of the lysin PlyV12. The results showed that the chimeric lysin Ply187N-V12C had not only lytic activity similar to Ply187N against staphylococcal strains but also extended its lytic activity to streptococci and enterococci, such as Streptococcus dysgalactiae, Streptococcus agalactiae, Streptococcus pyogenes, Enterococcus faecium and Enterococcus faecalis, which Ply187N could not lyse. Our work demonstrated that generating novel chimeric lysins with an extended lytic spectrum was feasible through fusing a catalytic domain with a cell-binding domain from lysins with lytic spectra across multiple genera.
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spelling pubmed-43533352015-03-12 Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci Dong, Qiuhua Wang, Jing Yang, Hang Wei, Cuihua Yu, Junping Zhang, Yun Huang, Yanling Zhang, Xian-En Wei, Hongping Microb Biotechnol Research Articles Developing chimeric lysins with a wide lytic spectrum would be important for treating some infections caused by multiple pathogenic bacteria. In the present work, a novel chimeric lysin (Ply187N-V12C) was constructed by fusing the catalytic domain (Ply187N) of the bacteriophage lysin Ply187 with the cell binding domain (146-314aa, V12C) of the lysin PlyV12. The results showed that the chimeric lysin Ply187N-V12C had not only lytic activity similar to Ply187N against staphylococcal strains but also extended its lytic activity to streptococci and enterococci, such as Streptococcus dysgalactiae, Streptococcus agalactiae, Streptococcus pyogenes, Enterococcus faecium and Enterococcus faecalis, which Ply187N could not lyse. Our work demonstrated that generating novel chimeric lysins with an extended lytic spectrum was feasible through fusing a catalytic domain with a cell-binding domain from lysins with lytic spectra across multiple genera. BlackWell Publishing Ltd 2015-03 2014-09-15 /pmc/articles/PMC4353335/ /pubmed/25219798 http://dx.doi.org/10.1111/1751-7915.12166 Text en Journal compilation © 2015 John Wiley & Sons Ltd and Society for Applied Microbiology http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Dong, Qiuhua
Wang, Jing
Yang, Hang
Wei, Cuihua
Yu, Junping
Zhang, Yun
Huang, Yanling
Zhang, Xian-En
Wei, Hongping
Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci
title Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci
title_full Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci
title_fullStr Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci
title_full_unstemmed Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci
title_short Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci
title_sort construction of a chimeric lysin ply187n-v12c with extended lytic activity against staphylococci and streptococci
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353335/
https://www.ncbi.nlm.nih.gov/pubmed/25219798
http://dx.doi.org/10.1111/1751-7915.12166
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