Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Respirometric profiling of isolated mitochondria is commonly used to investigate electron transport chain function. We describe a method for obtaining samples of human Vastus lateralis, isolating mitochondria from minimal amounts of skeletal muscle tissue, and plate based respirometric profiling usi...

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Detalles Bibliográficos
Autores principales: Bharadwaj, Manish S., Tyrrell, Daniel J., Lyles, Mary F., Demons, Jamehl L., Rogers, George W., Molina, Anthony J. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354623/
https://www.ncbi.nlm.nih.gov/pubmed/25741892
http://dx.doi.org/10.3791/52350

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354623/
https://www.ncbi.nlm.nih.gov/pubmed/25741892
http://dx.doi.org/10.3791/52350

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