An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

Here we describe a protocol to generate a co-culture consisting of 2 different neuronal populations. Induced pluripotent stem cells (iPSCs) are reprogrammed from human fibroblasts using episomal vectors. Colonies of iPSCs can be observed 30 days after initiation of fibroblast reprogramming. Pluripot...

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Detalles Bibliográficos
Autores principales: Su, Colin T. E., Yoon, Su-In, Marcy, Guillaume, Chin, Eunice W. M., Augustine, George J., Goh, Eyleen L. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354644/
https://www.ncbi.nlm.nih.gov/pubmed/25742527
http://dx.doi.org/10.3791/52408

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354644/
https://www.ncbi.nlm.nih.gov/pubmed/25742527
http://dx.doi.org/10.3791/52408

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