Cargando…

SwiftLib: rapid degenerate-codon-library optimization through dynamic programming

Degenerate codon (DC) libraries efficiently address the experimental library-size limitations of directed evolution by focusing diversity toward the positions and toward the amino acids (AAs) that are most likely to generate hits; however, manually constructing DC libraries is challenging, error pro...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jacobs, Timothy M., Yumerefendi, Hayretin, Kuhlman, Brian, Leaver-Fay, Andrew
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2015
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357694/ https://www.ncbi.nlm.nih.gov/pubmed/25539925 http://dx.doi.org/10.1093/nar/gku1323

Ejemplares similares

Four-base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids
por: Muranaka, Norihito, et al.
Publicado: (2006)

PaperClip: rapid multi-part DNA assembly from existing libraries
por: Trubitsyna, Maryia, et al.
Publicado: (2014)

The translation of recombinant proteins in E. coli can be improved by in silico generating and screening random libraries of a −70/+96 mRNA region with respect to the translation initiation codon
por: Care, S., et al.
Publicado: (2008)

A library-based method to rapidly analyse chromatin accessibility at multiple genomic regions
por: Basheer, Asjad, et al.
Publicado: (2009)

An ultra-dense library resource for rapid deconvolution of mutations that cause phenotypes in Escherichia coli
por: Nehring, Ralf B., et al.
Publicado: (2016)

Light-induced nuclear export reveals rapid dynamics of epigenetic modifications
por: Yumerefendi, Hayretin, et al.
Publicado: (2016)

Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP)
por: Hoskins, Roger A., et al.
Publicado: (2005)

A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries
por: Yates, Joshua D, et al.
Publicado: (2021)

Optimizing nucleotide sequence ensembles for combinatorial protein libraries using a genetic algorithm
por: Craig, Roger A., et al.
Publicado: (2010)

An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments
por: Heyer, Erin E., et al.
Publicado: (2015)

Noncanonical amino acid mutagenesis in response to recoding signal-enhanced quadruplet codons
por: Chen, Yan, et al.
Publicado: (2022)

An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptor
por: Dirnberger, Dietmar, et al.
Publicado: (2008)

Codon choice in genes depends on flanking sequence information—implications for theoretical reverse translation
por: Sivaraman, Karthikeyan, et al.
Publicado: (2008)

TrimerDimer: an oligonucleotide-based saturation mutagenesis approach that removes redundant and stop codons
por: Gaytán, Paul, et al.
Publicado: (2009)

A CUG codon adapted two-hybrid system for the pathogenic fungus Candida albicans
por: Stynen, Bram, et al.
Publicado: (2010)

Decomposition of time-dependent fluorescence signals reveals codon-specific kinetics of protein synthesis
por: Haase, Nadin, et al.
Publicado: (2018)

Site-specific chromosomal integration of large synthetic constructs
por: Kuhlman, Thomas E., et al.
Publicado: (2010)

Targeting individual cells by barcode in pooled sequence libraries
por: Ranu, Navpreet, et al.
Publicado: (2019)

A ‘poly-transfection’ method for rapid, one-pot characterization and optimization of genetic systems
por: Gam, Jeremy J, et al.
Publicado: (2019)

Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system
por: Yang, Fang, et al.
Publicado: (2018)

The use of multiple displacement amplification to amplify complex DNA libraries
por: Fullwood, Melissa J., et al.
Publicado: (2008)

Selection of functional human antibodies from retroviral display libraries
por: Urban, Johannes H., et al.
Publicado: (2005)

Integrase-directed recovery of functional genes from genomic libraries
por: Rowe-Magnus, Dean A.
Publicado: (2009)

A transposase strategy for creating libraries of circularly permuted proteins
por: Mehta, Manan M., et al.
Publicado: (2012)

Phage display peptide libraries: deviations from randomness and correctives
por: Ryvkin, Arie, et al.
Publicado: (2018)

Flexible CRISPR library construction using parallel oligonucleotide retrieval
por: Read, Abigail, et al.
Publicado: (2017)

Extensive libraries of gene truncation variants generated by in vitro transposition
por: Morelli, Aleardo, et al.
Publicado: (2017)

Targeted insertional mutagenesis libraries for deep domain insertion profiling
por: Coyote-Maestas, Willow, et al.
Publicado: (2020)

Integrating LibGuides into library websites
por: Dobbs, Aaron W, et al.
Publicado: (2016)

Promoter library designed for fine-tuned gene expression in Pichia pastoris
por: Hartner, Franz S., et al.
Publicado: (2008)

Covalent antibody display—an in vitro antibody-DNA library selection system
por: Reiersen, Herald, et al.
Publicado: (2005)

An on-bead tailing/ligation approach for sequencing resin-bound RNA libraries
por: Wiesmayr, Anna, et al.
Publicado: (2012)

Synthesis and cell-free cloning of DNA libraries using programmable microfluidics
por: Yehezkel, Tuval Ben, et al.
Publicado: (2016)

Blocking of targeted microRNAs from next-generation sequencing libraries
por: Roberts, Brian S., et al.
Publicado: (2015)

A platform for functional assessment of large variant libraries in mammalian cells
por: Matreyek, Kenneth A., et al.
Publicado: (2017)

Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery
por: Xu, Peng, et al.
Publicado: (2020)

An improved platform for functional assessment of large protein libraries in mammalian cells
por: Matreyek, Kenneth A, et al.
Publicado: (2020)

FLAG assay as a novel method for real-time signal generation during PCR: application to detection and genotyping of KRAS codon 12 mutations
por: Amicarelli, Giulia, et al.
Publicado: (2007)

Rapid and quantitative detection of homologous and non-homologous recombination events using three oligonucleotide MLPA
por: Langerak, Petra, et al.
Publicado: (2005)

One step construction of PCR mutagenized libraries for genetic analysis by recombination cloning
por: Khalil, Al-Muataz, et al.
Publicado: (2007)

Cannot write session to /tmp/vufind_sessions/sess_p41hr71dl97c4r7i8pbspbce80