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Generation and Developmental Characteristics of Porcine Tetraploid Embryos and Tetraploid/diploid Chimeric Embryos

The aim of this study was to optimize electrofusion conditions for generating porcine tetraploid (4n) embryos and produce tetraploid/diploid (4n/2n) chimeric embryos. Different electric field intensities were tested and 2 direct current (DC) pulses of 0.9 kV/cm for 30 μs was selected as the optimum...

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Detalles Bibliográficos
Autores principales: He, Wenteng, Kong, Qingran, Shi, Yongqian, Xie, Bingteng, Jiao, Mingxia, Huang, Tianqing, Guo, Shimeng, Hu, Kui, Liu, Zhonghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4357820/
https://www.ncbi.nlm.nih.gov/pubmed/24120753
http://dx.doi.org/10.1016/j.gpb.2013.09.007