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Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system

BACKGROUND: Two overlapping genes lacL and lacM (lacLM) encoding for heterodimeric β-galactosidase from Lactobacillus reuteri were previously cloned and over-expressed in the food-grade host strain Lactobacillus plantarum WCFS1, using the inducible lactobacillal pSIP expression system. In this study...

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Detalles Bibliográficos
Autores principales: Nguyen, Tien-Thanh, Nguyen, Hoang-Minh, Geiger, Barbara, Mathiesen, Geir, Eijsink, Vincent GH, Peterbauer, Clemens K, Haltrich, Dietmar, Nguyen, Thu-Ha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358714/
https://www.ncbi.nlm.nih.gov/pubmed/25880197
http://dx.doi.org/10.1186/s12934-015-0214-8