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Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system
BACKGROUND: Two overlapping genes lacL and lacM (lacLM) encoding for heterodimeric β-galactosidase from Lactobacillus reuteri were previously cloned and over-expressed in the food-grade host strain Lactobacillus plantarum WCFS1, using the inducible lactobacillal pSIP expression system. In this study...
Autores principales: | Nguyen, Tien-Thanh, Nguyen, Hoang-Minh, Geiger, Barbara, Mathiesen, Geir, Eijsink, Vincent GH, Peterbauer, Clemens K, Haltrich, Dietmar, Nguyen, Thu-Ha |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358714/ https://www.ncbi.nlm.nih.gov/pubmed/25880197 http://dx.doi.org/10.1186/s12934-015-0214-8 |
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