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Quantification of Mature MicroRNAs Using Pincer Probes and Real-Time PCR Amplification

The robust and reliable detection of small microRNAs (miRNAs) is important to understand the functional significance of miRNAs. Several methods can be used to quantify miRNAs. Selectively quantifying mature miRNAs among miRNA precursors, pri-miRNAs, and other miRNA-like sequences is challenging beca...

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Detalles Bibliográficos
Autores principales: Huang, Tinghua, Yang, Jun, Liu, Guopin, Jin, Wei, Liu, Zhi, Zhao, Shuhong, Yao, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359002/
https://www.ncbi.nlm.nih.gov/pubmed/25768430
http://dx.doi.org/10.1371/journal.pone.0120160