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Quantification of Mature MicroRNAs Using Pincer Probes and Real-Time PCR Amplification
The robust and reliable detection of small microRNAs (miRNAs) is important to understand the functional significance of miRNAs. Several methods can be used to quantify miRNAs. Selectively quantifying mature miRNAs among miRNA precursors, pri-miRNAs, and other miRNA-like sequences is challenging beca...
Autores principales: | Huang, Tinghua, Yang, Jun, Liu, Guopin, Jin, Wei, Liu, Zhi, Zhao, Shuhong, Yao, Min |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4359002/ https://www.ncbi.nlm.nih.gov/pubmed/25768430 http://dx.doi.org/10.1371/journal.pone.0120160 |
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