Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli

PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli. Although this gap-repair cloning approach is straightforward, its existence is virtually unknown to most molecular biologists. To popularize t...

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Detalles Bibliográficos
Autores principales: Jacobus, Ana Paula, Gross, Jeferson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361335/
https://www.ncbi.nlm.nih.gov/pubmed/25774528
http://dx.doi.org/10.1371/journal.pone.0119221

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361335/
https://www.ncbi.nlm.nih.gov/pubmed/25774528
http://dx.doi.org/10.1371/journal.pone.0119221

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