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Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli
PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli. Although this gap-repair cloning approach is straightforward, its existence is virtually unknown to most molecular biologists. To popularize t...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361335/ https://www.ncbi.nlm.nih.gov/pubmed/25774528 http://dx.doi.org/10.1371/journal.pone.0119221 |