Liquid-Based Iterative Recombineering Method Tolerant to Counter-Selection Escapes

Selection-based recombineering is a flexible and proven technology to precisely modify bacterial genomes at single base resolution. It consists of two steps of homologous recombination followed by selection/counter-selection. However, the shortage of efficient counter-selectable markers limits the t...

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Detalles Bibliográficos
Autores principales: Tominaga, Masahiro, Kawai-Noma, Shigeko, Kawagishi, Ikuro, Sowa, Yoshiyuki, Saito, Kyoichi, Umeno, Daisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361647/
https://www.ncbi.nlm.nih.gov/pubmed/25775434
http://dx.doi.org/10.1371/journal.pone.0119818

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361647/
https://www.ncbi.nlm.nih.gov/pubmed/25775434
http://dx.doi.org/10.1371/journal.pone.0119818

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