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Liquid-Based Iterative Recombineering Method Tolerant to Counter-Selection Escapes
Selection-based recombineering is a flexible and proven technology to precisely modify bacterial genomes at single base resolution. It consists of two steps of homologous recombination followed by selection/counter-selection. However, the shortage of efficient counter-selectable markers limits the t...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361647/ https://www.ncbi.nlm.nih.gov/pubmed/25775434 http://dx.doi.org/10.1371/journal.pone.0119818 |