High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins

Endo-β-N-acetylglucosaminidase H (Endo H, EC3.2.1.96) is a glycohydrolase that is widely used in the study of glycoproteins. The present study aimed to assess the effect of high-level endo-β-N-acetylglucosaminidase H expression in Pichia pastoris. The DNA coding sequence of this enzyme was optimized...

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Detalles Bibliográficos
Autores principales: Wang, Fei, Wang, Xiaojuan, Yu, Xiaolan, Fu, Lin, Liu, Yunyun, Ma, Lixin, Zhai, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362766/
https://www.ncbi.nlm.nih.gov/pubmed/25781897
http://dx.doi.org/10.1371/journal.pone.0120458

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4362766/
https://www.ncbi.nlm.nih.gov/pubmed/25781897
http://dx.doi.org/10.1371/journal.pone.0120458

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