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Mitofusin 2-Deficiency Suppresses Cell Proliferation through Disturbance of Autophagy
Mitofusin2 (Mfn2), a mitochondrial outer membrane protein serving primarily as a mitochondrial fusion protein, has multiple functions in regulating cell biological processes. Defects of Mfn2 were found in diabetes, obesity, and neurodegenerative diseases. In the present study, we found that knockdow...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363693/ https://www.ncbi.nlm.nih.gov/pubmed/25781899 http://dx.doi.org/10.1371/journal.pone.0121328 |
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author | Ding, Yanhong Gao, Han Zhao, Lifang Wang, Xian Zheng, Ming |
author_facet | Ding, Yanhong Gao, Han Zhao, Lifang Wang, Xian Zheng, Ming |
author_sort | Ding, Yanhong |
collection | PubMed |
description | Mitofusin2 (Mfn2), a mitochondrial outer membrane protein serving primarily as a mitochondrial fusion protein, has multiple functions in regulating cell biological processes. Defects of Mfn2 were found in diabetes, obesity, and neurodegenerative diseases. In the present study, we found that knockdown of Mfn2 by shRNA led to impaired autophagic degradation, inhibited mitochondrial oxygen consumption rate and cell glycolysis, reduced ATP production, and suppressed cell proliferation. Inhibition of autophagic degradation mimicked Mfn2-deficiency mediated cell proliferation suppression, while enhancement of autophagosome maturation restored the suppressed cell proliferation by Mfn2-deficiency. Thus, our findings revealed the role of Mfn2 in regulating cell proliferation and mitochondrial metabolism, and shed new light on understanding the mechanisms of Mfn2 deficiency related diseases. |
format | Online Article Text |
id | pubmed-4363693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43636932015-03-23 Mitofusin 2-Deficiency Suppresses Cell Proliferation through Disturbance of Autophagy Ding, Yanhong Gao, Han Zhao, Lifang Wang, Xian Zheng, Ming PLoS One Research Article Mitofusin2 (Mfn2), a mitochondrial outer membrane protein serving primarily as a mitochondrial fusion protein, has multiple functions in regulating cell biological processes. Defects of Mfn2 were found in diabetes, obesity, and neurodegenerative diseases. In the present study, we found that knockdown of Mfn2 by shRNA led to impaired autophagic degradation, inhibited mitochondrial oxygen consumption rate and cell glycolysis, reduced ATP production, and suppressed cell proliferation. Inhibition of autophagic degradation mimicked Mfn2-deficiency mediated cell proliferation suppression, while enhancement of autophagosome maturation restored the suppressed cell proliferation by Mfn2-deficiency. Thus, our findings revealed the role of Mfn2 in regulating cell proliferation and mitochondrial metabolism, and shed new light on understanding the mechanisms of Mfn2 deficiency related diseases. Public Library of Science 2015-03-17 /pmc/articles/PMC4363693/ /pubmed/25781899 http://dx.doi.org/10.1371/journal.pone.0121328 Text en © 2015 Ding et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ding, Yanhong Gao, Han Zhao, Lifang Wang, Xian Zheng, Ming Mitofusin 2-Deficiency Suppresses Cell Proliferation through Disturbance of Autophagy |
title | Mitofusin 2-Deficiency Suppresses Cell Proliferation through Disturbance of Autophagy |
title_full | Mitofusin 2-Deficiency Suppresses Cell Proliferation through Disturbance of Autophagy |
title_fullStr | Mitofusin 2-Deficiency Suppresses Cell Proliferation through Disturbance of Autophagy |
title_full_unstemmed | Mitofusin 2-Deficiency Suppresses Cell Proliferation through Disturbance of Autophagy |
title_short | Mitofusin 2-Deficiency Suppresses Cell Proliferation through Disturbance of Autophagy |
title_sort | mitofusin 2-deficiency suppresses cell proliferation through disturbance of autophagy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363693/ https://www.ncbi.nlm.nih.gov/pubmed/25781899 http://dx.doi.org/10.1371/journal.pone.0121328 |
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