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Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers

BACKGROUND: A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers. FINDINGS: Species specificity was tested with a panel of 13 Burkholderia ty...

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Detalles Bibliográficos
Autores principales: Schmoock, Gernot, Elschner, Mandy, Sprague, Lisa D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364355/
https://www.ncbi.nlm.nih.gov/pubmed/25887130
http://dx.doi.org/10.1186/s13028-015-0104-4