Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers

BACKGROUND: A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers. FINDINGS: Species specificity was tested with a panel of 13 Burkholderia ty...

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Autores principales: Schmoock, Gernot, Elschner, Mandy, Sprague, Lisa D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364355/
https://www.ncbi.nlm.nih.gov/pubmed/25887130
http://dx.doi.org/10.1186/s13028-015-0104-4
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author Schmoock, Gernot
Elschner, Mandy
Sprague, Lisa D
author_facet Schmoock, Gernot
Elschner, Mandy
Sprague, Lisa D
author_sort Schmoock, Gernot
collection PubMed
description BACKGROUND: A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers. FINDINGS: Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. thailandensis field strain from different geographic regions were tested and correctly identified. Testing of 55 non-Burkholderia bacterial species revealed 100% specificity of the assay. The minimum detection limit was 1 pg DNA or 160 GE for B. mallei and 130 GE for B. pseudomallei, respectively. CONCLUSIONS: This assay enables the clear distinction between B. mallei and B. pseudomallei/B. thailandensis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13028-015-0104-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-43643552015-03-19 Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers Schmoock, Gernot Elschner, Mandy Sprague, Lisa D Acta Vet Scand Brief Communication BACKGROUND: A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers. FINDINGS: Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. thailandensis field strain from different geographic regions were tested and correctly identified. Testing of 55 non-Burkholderia bacterial species revealed 100% specificity of the assay. The minimum detection limit was 1 pg DNA or 160 GE for B. mallei and 130 GE for B. pseudomallei, respectively. CONCLUSIONS: This assay enables the clear distinction between B. mallei and B. pseudomallei/B. thailandensis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13028-015-0104-4) contains supplementary material, which is available to authorized users. BioMed Central 2015-03-07 /pmc/articles/PMC4364355/ /pubmed/25887130 http://dx.doi.org/10.1186/s13028-015-0104-4 Text en © Schmoock et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Brief Communication
Schmoock, Gernot
Elschner, Mandy
Sprague, Lisa D
Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers
title Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers
title_full Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers
title_fullStr Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers
title_full_unstemmed Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers
title_short Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers
title_sort clear distinction between burkholderia mallei and burkholderia pseudomallei using fluorescent motb primers
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364355/
https://www.ncbi.nlm.nih.gov/pubmed/25887130
http://dx.doi.org/10.1186/s13028-015-0104-4
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