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Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel

BACKGROUND: We previously cloned a 1,3-specific lipase gene from the fungus Rhizomucor miehei and expressed it in methylotrophic yeast Pichia pastoris strain GS115. The enzyme produced (termed RML) was able to catalyze methanolysis of soybean oil and showed strong position specificity. However, the...

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Detalles Bibliográficos
Autores principales: Huang, Jinjin, Xia, Ji, Yang, Zhen, Guan, Feifei, Cui, Di, Guan, Guohua, Jiang, Wei, Li, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364654/
https://www.ncbi.nlm.nih.gov/pubmed/25788976
http://dx.doi.org/10.1186/1754-6834-7-111