Direct Determination of Phosphatase Activity from Physiological Substrates in Cells

A direct and continuous approach to determine simultaneously protein and phosphate concentrations in cells and kinetics of phosphate release from physiological substrates by cells without any labeling has been developed. Among the enzymes having a phosphatase activity, tissue non-specific alkaline p...

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Detalles Bibliográficos
Autores principales: Ren, Zhongyuan, Do, Le Duy, Bechkoff, Géraldine, Mebarek, Saida, Keloglu, Nermin, Ahamada, Saandia, Meena, Saurabh, Magne, David, Pikula, Slawomir, Wu, Yuqing, Buchet, René
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364917/
https://www.ncbi.nlm.nih.gov/pubmed/25785438
http://dx.doi.org/10.1371/journal.pone.0120087

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364917/
https://www.ncbi.nlm.nih.gov/pubmed/25785438
http://dx.doi.org/10.1371/journal.pone.0120087

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