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Direct Determination of Phosphatase Activity from Physiological Substrates in Cells
A direct and continuous approach to determine simultaneously protein and phosphate concentrations in cells and kinetics of phosphate release from physiological substrates by cells without any labeling has been developed. Among the enzymes having a phosphatase activity, tissue non-specific alkaline p...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4364917/ https://www.ncbi.nlm.nih.gov/pubmed/25785438 http://dx.doi.org/10.1371/journal.pone.0120087 |