DICER-ARGONAUTE2 Complex in Continuous Fluorogenic Assays of RNA Interference Enzymes

Mechanistic studies of RNA processing in the RNA-Induced Silencing Complex (RISC) have been hindered by lack of methods for continuous monitoring of enzymatic activity. “Quencherless” fluorogenic substrates of RNAi enzymes enable continuous monitoring of enzymatic reactions for detailed kinetics stu...

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Detalles Bibliográficos
Autores principales: Bernard, Mark A., Wang, Leyu, Tachado, Souvenir D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368098/
https://www.ncbi.nlm.nih.gov/pubmed/25793518
http://dx.doi.org/10.1371/journal.pone.0120614

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368098/
https://www.ncbi.nlm.nih.gov/pubmed/25793518
http://dx.doi.org/10.1371/journal.pone.0120614

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