Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology

The aim of this study was to develop a simple and reliable high-performance chromatographic (HPLC) method for simultaneous analysis of adapalene and benzoyl peroxide in pharmaceutical formulation by response surface methodology (RSM). An optimized mobile phase composed of acetonitrile, tetrahydrofur...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Yi-Cheng, Tsai, Pi-Ju, Huang, Yaw-Bin, Wu, Pao-Chu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368549/
https://www.ncbi.nlm.nih.gov/pubmed/25793581
http://dx.doi.org/10.1371/journal.pone.0120171
_version_ 1782362637772783616
author Chen, Yi-Cheng
Tsai, Pi-Ju
Huang, Yaw-Bin
Wu, Pao-Chu
author_facet Chen, Yi-Cheng
Tsai, Pi-Ju
Huang, Yaw-Bin
Wu, Pao-Chu
author_sort Chen, Yi-Cheng
collection PubMed
description The aim of this study was to develop a simple and reliable high-performance chromatographic (HPLC) method for simultaneous analysis of adapalene and benzoyl peroxide in pharmaceutical formulation by response surface methodology (RSM). An optimized mobile phase composed of acetonitrile, tetrahydrofuran and water containing 0.1% acetic acid at a ratio of 25:50:25 by volume was successfully predicted by using RSM. An isocratic separation was achieved by using the condition. Furthermore, the analytical method was validated in terms of specificity, linearity, accuracy and precision in a range of 80% to 120% of the expected concentration. Finally, the method was successfully applied to the analysis of a commercial product.
format Online
Article
Text
id pubmed-4368549
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-43685492015-03-27 Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology Chen, Yi-Cheng Tsai, Pi-Ju Huang, Yaw-Bin Wu, Pao-Chu PLoS One Research Article The aim of this study was to develop a simple and reliable high-performance chromatographic (HPLC) method for simultaneous analysis of adapalene and benzoyl peroxide in pharmaceutical formulation by response surface methodology (RSM). An optimized mobile phase composed of acetonitrile, tetrahydrofuran and water containing 0.1% acetic acid at a ratio of 25:50:25 by volume was successfully predicted by using RSM. An isocratic separation was achieved by using the condition. Furthermore, the analytical method was validated in terms of specificity, linearity, accuracy and precision in a range of 80% to 120% of the expected concentration. Finally, the method was successfully applied to the analysis of a commercial product. Public Library of Science 2015-03-20 /pmc/articles/PMC4368549/ /pubmed/25793581 http://dx.doi.org/10.1371/journal.pone.0120171 Text en © 2015 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Yi-Cheng
Tsai, Pi-Ju
Huang, Yaw-Bin
Wu, Pao-Chu
Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology
title Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology
title_full Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology
title_fullStr Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology
title_full_unstemmed Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology
title_short Optimization and Validation of High-Performance Chromatographic Condition for Simultaneous Determination of Adapalene and Benzoyl Peroxide by Response Surface Methodology
title_sort optimization and validation of high-performance chromatographic condition for simultaneous determination of adapalene and benzoyl peroxide by response surface methodology
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4368549/
https://www.ncbi.nlm.nih.gov/pubmed/25793581
http://dx.doi.org/10.1371/journal.pone.0120171
work_keys_str_mv AT chenyicheng optimizationandvalidationofhighperformancechromatographicconditionforsimultaneousdeterminationofadapaleneandbenzoylperoxidebyresponsesurfacemethodology
AT tsaipiju optimizationandvalidationofhighperformancechromatographicconditionforsimultaneousdeterminationofadapaleneandbenzoylperoxidebyresponsesurfacemethodology
AT huangyawbin optimizationandvalidationofhighperformancechromatographicconditionforsimultaneousdeterminationofadapaleneandbenzoylperoxidebyresponsesurfacemethodology
AT wupaochu optimizationandvalidationofhighperformancechromatographicconditionforsimultaneousdeterminationofadapaleneandbenzoylperoxidebyresponsesurfacemethodology

Ejemplares similares