Cargando…
Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids
Flavonoids were found previously to modulate efficiency of synthesis of glycosaminoglycans (GAGs), compounds which are accumulated in cells of patients suffering from mucopolysaccharidoses (MPSs). The aim of this work was to determine effects of different flavonoids (genistein, kaempferol, daidzein)...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369724/ https://www.ncbi.nlm.nih.gov/pubmed/25797591 http://dx.doi.org/10.1038/srep09378 |
_version_ | 1782362781760094208 |
---|---|
author | Moskot, Marta Jakóbkiewicz-Banecka, Joanna Kloska, Anna Smolińska, Elwira Mozolewski, Paweł Malinowska, Marcelina Rychłowski, Michał Banecki, Bogdan Węgrzyn, Grzegorz Gabig-Cimińska, Magdalena |
author_facet | Moskot, Marta Jakóbkiewicz-Banecka, Joanna Kloska, Anna Smolińska, Elwira Mozolewski, Paweł Malinowska, Marcelina Rychłowski, Michał Banecki, Bogdan Węgrzyn, Grzegorz Gabig-Cimińska, Magdalena |
author_sort | Moskot, Marta |
collection | PubMed |
description | Flavonoids were found previously to modulate efficiency of synthesis of glycosaminoglycans (GAGs), compounds which are accumulated in cells of patients suffering from mucopolysaccharidoses (MPSs). The aim of this work was to determine effects of different flavonoids (genistein, kaempferol, daidzein) used alone or in combinations, on expression of genes coding for proteins involved in GAG metabolism. Analyses with DNA microarray, followed by real-time qRT-PCR revealed that genistein, kaempferol and combination of these two compounds induced dose- and time-dependent remarkable alterations in transcript profiles of GAG metabolism genes in cultures of wild-type human dermal fibroblasts (HDFa). Interestingly, effects of the mixture of genistein and kaempferol were stronger than those revealed by any of these compounds used alone. Similarly, the most effective reduction in levels of GAG production, in both HDFa and MPS II cells, was observed in the presence of genistein, keampferol and combination of these compounds. Forty five genes were chosen for further verification not only in HDFa, but also in MPS II fibroblasts by using real-time qRT-PCR. Despite effects on GAG metabolism-related genes, we found that genistein, kaempferol and mixture of these compounds significantly stimulated expression of TFEB. Additionally, a decrease in MTOR transcript level was observed at these conditions. |
format | Online Article Text |
id | pubmed-4369724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43697242015-04-06 Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids Moskot, Marta Jakóbkiewicz-Banecka, Joanna Kloska, Anna Smolińska, Elwira Mozolewski, Paweł Malinowska, Marcelina Rychłowski, Michał Banecki, Bogdan Węgrzyn, Grzegorz Gabig-Cimińska, Magdalena Sci Rep Article Flavonoids were found previously to modulate efficiency of synthesis of glycosaminoglycans (GAGs), compounds which are accumulated in cells of patients suffering from mucopolysaccharidoses (MPSs). The aim of this work was to determine effects of different flavonoids (genistein, kaempferol, daidzein) used alone or in combinations, on expression of genes coding for proteins involved in GAG metabolism. Analyses with DNA microarray, followed by real-time qRT-PCR revealed that genistein, kaempferol and combination of these two compounds induced dose- and time-dependent remarkable alterations in transcript profiles of GAG metabolism genes in cultures of wild-type human dermal fibroblasts (HDFa). Interestingly, effects of the mixture of genistein and kaempferol were stronger than those revealed by any of these compounds used alone. Similarly, the most effective reduction in levels of GAG production, in both HDFa and MPS II cells, was observed in the presence of genistein, keampferol and combination of these compounds. Forty five genes were chosen for further verification not only in HDFa, but also in MPS II fibroblasts by using real-time qRT-PCR. Despite effects on GAG metabolism-related genes, we found that genistein, kaempferol and mixture of these compounds significantly stimulated expression of TFEB. Additionally, a decrease in MTOR transcript level was observed at these conditions. Nature Publishing Group 2015-03-23 /pmc/articles/PMC4369724/ /pubmed/25797591 http://dx.doi.org/10.1038/srep09378 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Moskot, Marta Jakóbkiewicz-Banecka, Joanna Kloska, Anna Smolińska, Elwira Mozolewski, Paweł Malinowska, Marcelina Rychłowski, Michał Banecki, Bogdan Węgrzyn, Grzegorz Gabig-Cimińska, Magdalena Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids |
title | Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids |
title_full | Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids |
title_fullStr | Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids |
title_full_unstemmed | Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids |
title_short | Modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids |
title_sort | modulation of expression of genes involved in glycosaminoglycan metabolism and lysosome biogenesis by flavonoids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369724/ https://www.ncbi.nlm.nih.gov/pubmed/25797591 http://dx.doi.org/10.1038/srep09378 |
work_keys_str_mv | AT moskotmarta modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids AT jakobkiewiczbaneckajoanna modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids AT kloskaanna modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids AT smolinskaelwira modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids AT mozolewskipaweł modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids AT malinowskamarcelina modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids AT rychłowskimichał modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids AT baneckibogdan modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids AT wegrzyngrzegorz modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids AT gabigciminskamagdalena modulationofexpressionofgenesinvolvedinglycosaminoglycanmetabolismandlysosomebiogenesisbyflavonoids |