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Development of a Molecularly Evolved, Highly Sensitive CaMKII FRET Sensor with Improved Expression Pattern

Genetically encoded fluorescence resonance energy transfer (FRET) biosensors have been successfully used to visualize protein activity in living cells. The sensitivity and accuracy of FRET measurements directly depend on biosensor folding efficiency, expression pattern, sensitivity, and dynamic rang...

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Detalles Bibliográficos
Autores principales: Shibata, Akihiro C. E., Maebashi, Hiroshi K., Nakahata, Yoshihisa, Nabekura, Junichi, Murakoshi, Hideji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370617/
https://www.ncbi.nlm.nih.gov/pubmed/25799407
http://dx.doi.org/10.1371/journal.pone.0121109