Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli

A gene encoding a galactose oxidase (GalOx) was isolated from Fusarium sambucinum cultures and overexpressed in Escherichia coli yielding 4.4 mg enzyme per L of growth culture with a specific activity of 159 U mg(−1). By adding a C-terminal His-tag the enzyme could be easily purified with a single a...

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Detalles Bibliográficos
Autores principales: Paukner, Regina, Staudigl, Petra, Choosri, Withu, Haltrich, Dietmar, Leitner, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370742/
https://www.ncbi.nlm.nih.gov/pubmed/25543085
http://dx.doi.org/10.1016/j.pep.2014.12.010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370742/
https://www.ncbi.nlm.nih.gov/pubmed/25543085
http://dx.doi.org/10.1016/j.pep.2014.12.010

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