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Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli
A gene encoding a galactose oxidase (GalOx) was isolated from Fusarium sambucinum cultures and overexpressed in Escherichia coli yielding 4.4 mg enzyme per L of growth culture with a specific activity of 159 U mg(−1). By adding a C-terminal His-tag the enzyme could be easily purified with a single a...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370742/ https://www.ncbi.nlm.nih.gov/pubmed/25543085 http://dx.doi.org/10.1016/j.pep.2014.12.010 |