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Auto-induction expression of human consensus interferon-alpha in Escherichia coli
BACKGROUND: Isopropyl-β-D-1-thiolgalactopyranoside (IPTG)-inducible expression of recombinant proteins in E. coli is commonly used and effective. Nevertheless, unintended induction was encountered as a problem when using these bacterial expression systems, generating cultures that give reduced or va...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372041/ https://www.ncbi.nlm.nih.gov/pubmed/25886839 http://dx.doi.org/10.1186/s12896-015-0128-x |