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Auto-induction expression of human consensus interferon-alpha in Escherichia coli

BACKGROUND: Isopropyl-β-D-1-thiolgalactopyranoside (IPTG)-inducible expression of recombinant proteins in E. coli is commonly used and effective. Nevertheless, unintended induction was encountered as a problem when using these bacterial expression systems, generating cultures that give reduced or va...

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Detalles Bibliográficos
Autores principales: EL-Baky, Nawal Abd, Linjawi, Mustafa H, Redwan, Elrashdy M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372041/
https://www.ncbi.nlm.nih.gov/pubmed/25886839
http://dx.doi.org/10.1186/s12896-015-0128-x