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Lambda Red Mediated Gap Repair Utilizes a Novel Replicative Intermediate in Escherichia coli

The lambda phage Red recombination system can mediate efficient homologous recombination in Escherichia coli, which is the basis of the DNA engineering technique termed recombineering. Red mediated insertion of DNA requires DNA replication, involves a single-stranded DNA intermediate and is more eff...

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Detalles Bibliográficos
Autores principales: Reddy, Thimma R., Fevat, Léna M. S., Munson, Sarah E., Stewart, A. Francis, Cowley, Shaun M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372340/
https://www.ncbi.nlm.nih.gov/pubmed/25803509
http://dx.doi.org/10.1371/journal.pone.0120681