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Lambda Red Mediated Gap Repair Utilizes a Novel Replicative Intermediate in Escherichia coli
The lambda phage Red recombination system can mediate efficient homologous recombination in Escherichia coli, which is the basis of the DNA engineering technique termed recombineering. Red mediated insertion of DNA requires DNA replication, involves a single-stranded DNA intermediate and is more eff...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372340/ https://www.ncbi.nlm.nih.gov/pubmed/25803509 http://dx.doi.org/10.1371/journal.pone.0120681 |