Cargando…
Lambda Red Mediated Gap Repair Utilizes a Novel Replicative Intermediate in Escherichia coli
The lambda phage Red recombination system can mediate efficient homologous recombination in Escherichia coli, which is the basis of the DNA engineering technique termed recombineering. Red mediated insertion of DNA requires DNA replication, involves a single-stranded DNA intermediate and is more eff...
Autores principales: | Reddy, Thimma R., Fevat, Léna M. S., Munson, Sarah E., Stewart, A. Francis, Cowley, Shaun M. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372340/ https://www.ncbi.nlm.nih.gov/pubmed/25803509 http://dx.doi.org/10.1371/journal.pone.0120681 |
Ejemplares similares
-
Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
por: Reddy, Thimma R., et al.
Publicado: (2015) -
Differential Requirements of Singleplex and Multiplex Recombineering of Large DNA Constructs
por: Reddy, Thimma R., et al.
Publicado: (2015) -
Involvement of Escherichia coli DNA Replication Proteins in Phage Lambda Red-Mediated Homologous Recombination
por: Poteete, Anthony R.
Publicado: (2013) -
Genomic landscape of single-stranded DNA gapped intermediates in Escherichia coli
por: Pham, Phuong, et al.
Publicado: (2021) -
Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases
por: Mosberg, Joshua A., et al.
Publicado: (2012)