Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system

Targeted transgenic mouse models, where an exogenous gene is inserted into a specified genomic locus to achieve its stable and reliable expression, have been widely used in biomedical research. However, the available methodologies for targeted insertion of sequences require many laborious steps that...

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Detalles Bibliográficos
Autores principales: Quadros, Rolen M., Harms, Donald W., Ohtsuka, Masato, Gurumurthy, Channabasavaiah B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372609/
https://www.ncbi.nlm.nih.gov/pubmed/25834785
http://dx.doi.org/10.1016/j.fob.2015.03.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4372609/
https://www.ncbi.nlm.nih.gov/pubmed/25834785
http://dx.doi.org/10.1016/j.fob.2015.03.003

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