Partially Overlapping Primer-Based PCR for Genome Walking

Current genome walking methods are cumbersome to perform and can result in non-specific products. Here, we demonstrate the use of partially overlapping primer-based PCR (POP-PCR), a direct genome walking technique for the isolation of unknown flanking regions. This method exploits the partially over...

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Detalles Bibliográficos
Autores principales: Li, Haixing, Ding, Dongqin, Cao, Yusheng, Yu, Bo, Guo, Liang, Liu, Xiaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374871/
https://www.ncbi.nlm.nih.gov/pubmed/25811779
http://dx.doi.org/10.1371/journal.pone.0120139

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4374871/
https://www.ncbi.nlm.nih.gov/pubmed/25811779
http://dx.doi.org/10.1371/journal.pone.0120139

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