A Method to Quantify FRET Stoichiometry with Phasor Plot Analysis and Acceptor Lifetime Ingrowth

FRET is widely used for the study of protein-protein interactions in biological samples. However, it is difficult to quantify both the FRET efficiency (E) and the affinity (K(d)) of the molecular interaction from intermolecular FRET signals in samples of unknown stoichiometry. Here, we present a met...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, WeiYue, Avezov, Edward, Schlachter, Simon C., Gielen, Fabrice, Laine, Romain F., Harding, Heather P., Hollfelder, Florian, Ron, David, Kaminski, Clemens F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2015
Materias:
Biophysical Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375440/
https://www.ncbi.nlm.nih.gov/pubmed/25762312
http://dx.doi.org/10.1016/j.bpj.2015.01.012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375440/
https://www.ncbi.nlm.nih.gov/pubmed/25762312
http://dx.doi.org/10.1016/j.bpj.2015.01.012

Ejemplares similares