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The Marine Metabolite SZ-685C Induces Apoptosis in Primary Human Nonfunctioning Pituitary Adenoma Cells by Inhibition of the Akt Pathway in Vitro

Nonfunctioning pituitary adenoma (NFPA) is one of the most common types of pituitary adenoma. The marine anthraquinone derivative SZ-685C has been isolated from the secondary metabolites of the mangrove endophytic fungus Halorosellinia sp. (No. 1403) which is found in the South China Sea. Recent res...

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Autores principales: Wang, Xin, Tan, Ting, Mao, Zhi-Gang, Lei, Ni, Wang, Zong-Ming, Hu, Bin, Chen, Zhi-Yong, She, Zhi-Gang, Zhu, Yong-Hong, Wang, Hai-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378000/
https://www.ncbi.nlm.nih.gov/pubmed/25806467
http://dx.doi.org/10.3390/md13031569
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author Wang, Xin
Tan, Ting
Mao, Zhi-Gang
Lei, Ni
Wang, Zong-Ming
Hu, Bin
Chen, Zhi-Yong
She, Zhi-Gang
Zhu, Yong-Hong
Wang, Hai-Jun
author_facet Wang, Xin
Tan, Ting
Mao, Zhi-Gang
Lei, Ni
Wang, Zong-Ming
Hu, Bin
Chen, Zhi-Yong
She, Zhi-Gang
Zhu, Yong-Hong
Wang, Hai-Jun
author_sort Wang, Xin
collection PubMed
description Nonfunctioning pituitary adenoma (NFPA) is one of the most common types of pituitary adenoma. The marine anthraquinone derivative SZ-685C has been isolated from the secondary metabolites of the mangrove endophytic fungus Halorosellinia sp. (No. 1403) which is found in the South China Sea. Recent research has shown that SZ-685C possesses anticancer and tumor suppressive effects. The tetrazolium-based colorimetric assay (MTT assay) to investigate the different effect of the marine compound SZ-685C on the proliferation of primary human NFPA cells, rat normal pituitary cells (RPCs) and rat prolactinoma MMQ cell lines. Hoechst 33342 dye/propidium iodide (PI) double staining and fluorescein isothiocyanate-conjugated Annexin V/PI (Annexin V-FITC/PI) apoptosis assays detected an enhanced rate of apoptosis in cells treated with SZ-685C. Enhanced expression levels of caspase 3 and phosphate and tensin homolog (PTEN) were determined by Western blotting. Notably, the protein expression levels of Akt were decreased when the primary human NFPA cells were treated with SZ-685C. Here, we show that SZ-685C induces apoptosis of human NFPA cells through inhibition of the Akt pathway in vitro. The understanding of apoptosis has provided the basis for novel targeted therapies that can induce death in cancer cells or sensitize them to established cytotoxic agents and radiation therapy.
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spelling pubmed-43780002015-04-27 The Marine Metabolite SZ-685C Induces Apoptosis in Primary Human Nonfunctioning Pituitary Adenoma Cells by Inhibition of the Akt Pathway in Vitro Wang, Xin Tan, Ting Mao, Zhi-Gang Lei, Ni Wang, Zong-Ming Hu, Bin Chen, Zhi-Yong She, Zhi-Gang Zhu, Yong-Hong Wang, Hai-Jun Mar Drugs Article Nonfunctioning pituitary adenoma (NFPA) is one of the most common types of pituitary adenoma. The marine anthraquinone derivative SZ-685C has been isolated from the secondary metabolites of the mangrove endophytic fungus Halorosellinia sp. (No. 1403) which is found in the South China Sea. Recent research has shown that SZ-685C possesses anticancer and tumor suppressive effects. The tetrazolium-based colorimetric assay (MTT assay) to investigate the different effect of the marine compound SZ-685C on the proliferation of primary human NFPA cells, rat normal pituitary cells (RPCs) and rat prolactinoma MMQ cell lines. Hoechst 33342 dye/propidium iodide (PI) double staining and fluorescein isothiocyanate-conjugated Annexin V/PI (Annexin V-FITC/PI) apoptosis assays detected an enhanced rate of apoptosis in cells treated with SZ-685C. Enhanced expression levels of caspase 3 and phosphate and tensin homolog (PTEN) were determined by Western blotting. Notably, the protein expression levels of Akt were decreased when the primary human NFPA cells were treated with SZ-685C. Here, we show that SZ-685C induces apoptosis of human NFPA cells through inhibition of the Akt pathway in vitro. The understanding of apoptosis has provided the basis for novel targeted therapies that can induce death in cancer cells or sensitize them to established cytotoxic agents and radiation therapy. MDPI 2015-03-23 /pmc/articles/PMC4378000/ /pubmed/25806467 http://dx.doi.org/10.3390/md13031569 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xin
Tan, Ting
Mao, Zhi-Gang
Lei, Ni
Wang, Zong-Ming
Hu, Bin
Chen, Zhi-Yong
She, Zhi-Gang
Zhu, Yong-Hong
Wang, Hai-Jun
The Marine Metabolite SZ-685C Induces Apoptosis in Primary Human Nonfunctioning Pituitary Adenoma Cells by Inhibition of the Akt Pathway in Vitro
title The Marine Metabolite SZ-685C Induces Apoptosis in Primary Human Nonfunctioning Pituitary Adenoma Cells by Inhibition of the Akt Pathway in Vitro
title_full The Marine Metabolite SZ-685C Induces Apoptosis in Primary Human Nonfunctioning Pituitary Adenoma Cells by Inhibition of the Akt Pathway in Vitro
title_fullStr The Marine Metabolite SZ-685C Induces Apoptosis in Primary Human Nonfunctioning Pituitary Adenoma Cells by Inhibition of the Akt Pathway in Vitro
title_full_unstemmed The Marine Metabolite SZ-685C Induces Apoptosis in Primary Human Nonfunctioning Pituitary Adenoma Cells by Inhibition of the Akt Pathway in Vitro
title_short The Marine Metabolite SZ-685C Induces Apoptosis in Primary Human Nonfunctioning Pituitary Adenoma Cells by Inhibition of the Akt Pathway in Vitro
title_sort marine metabolite sz-685c induces apoptosis in primary human nonfunctioning pituitary adenoma cells by inhibition of the akt pathway in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378000/
https://www.ncbi.nlm.nih.gov/pubmed/25806467
http://dx.doi.org/10.3390/md13031569
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