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Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins

We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer superior structural preservation, performing in-resin super-resolution, however, remains a challenge....

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Detalles Bibliográficos
Autores principales: Johnson, Errin, Seiradake, Elena, Jones, E. Yvonne, Davis, Ilan, Grünewald, Kay, Kaufmann, Rainer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379466/
https://www.ncbi.nlm.nih.gov/pubmed/25823571
http://dx.doi.org/10.1038/srep09583