Detection of Ochratoxin a Using Molecular Beacons and Real-Time PCR Thermal Cycler

We developed a simple and cheap assay for quantitatively detecting ochratoxin A (OTA) in wine. A DNA aptamer available in literature was used as recognition probe in its molecular beacon form, i.e., with a fluorescence-quenching pair at the stem ends. Our aptabeacon could adopt a conformation allowi...

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Detalles Bibliográficos
Autores principales: Sanzani, Simona Marianna, Reverberi, Massimo, Fanelli, Corrado, Ippolito, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379526/
https://www.ncbi.nlm.nih.gov/pubmed/25760080
http://dx.doi.org/10.3390/toxins7030812

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379526/
https://www.ncbi.nlm.nih.gov/pubmed/25760080
http://dx.doi.org/10.3390/toxins7030812

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