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FlowClus: efficiently filtering and denoising pyrosequenced amplicons

BACKGROUND: Reducing the effects of sequencing errors and PCR artifacts has emerged as an essential component in amplicon-based metagenomic studies. Denoising algorithms have been designed that can reduce error rates in mock community data, but they change the sequence data in a manner that can be i...

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Detalles Bibliográficos
Autores principales: Gaspar, John M, Thomas, W Kelley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4380255/
https://www.ncbi.nlm.nih.gov/pubmed/25885646
http://dx.doi.org/10.1186/s12859-015-0532-1