Single molecule TPM analysis of the catalytic pentad mutants of Cre and Flp site-specific recombinases: contributions of the pentad residues to the pre-chemical steps of recombination

Cre and Flp site-specific recombinase variants harboring point mutations at their conserved catalytic pentad positions were characterized using single molecule tethered particle motion (TPM) analysis. The findings reveal contributions of these amino acids to the pre-chemical steps of recombination....

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Detalles Bibliográficos
Autores principales: Fan, Hsiu-Fang, Cheng, Yong-Song, Ma, Chien-Hui, Jayaram, Makkuni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381057/
https://www.ncbi.nlm.nih.gov/pubmed/25765648
http://dx.doi.org/10.1093/nar/gkv114

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381057/
https://www.ncbi.nlm.nih.gov/pubmed/25765648
http://dx.doi.org/10.1093/nar/gkv114

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