A hybrid chimeric system for versatile and ultra-sensitive RNase detection

We developed a new versatile strategy that allows the detection of several classes of RNases (i.e., targeting ss- or ds-RNA, DNA/RNA hetero-hybrid or junctions) with higher sensitivity than existing assays. Our two-step approach consists of a DNA-RNA-DNA chimeric Hairpin Probe (cHP) conjugated to ma...

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Detalles Bibliográficos
Autores principales: Persano, Stefano, Vecchio, Giuseppe, Pompa, Pier Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381352/
https://www.ncbi.nlm.nih.gov/pubmed/25828752
http://dx.doi.org/10.1038/srep09558

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381352/
https://www.ncbi.nlm.nih.gov/pubmed/25828752
http://dx.doi.org/10.1038/srep09558

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