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Printing 2-Dimentional Droplet Array for Single-Cell Reverse Transcription Quantitative PCR Assay with a Microfluidic Robot

This paper describes a nanoliter droplet array-based single-cell reverse transcription quantitative PCR (RT-qPCR) assay method for quantifying gene expression in individual cells. By sequentially printing nanoliter-scale droplets on microchip using a microfluidic robot, all liquid-handling operation...

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Detalles Bibliográficos
Autores principales: Zhu, Ying, Zhang, Yun-Xia, Liu, Wen-Wen, Ma, Yan, Fang, Qun, Yao, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4381353/
https://www.ncbi.nlm.nih.gov/pubmed/25828383
http://dx.doi.org/10.1038/srep09551