Ultrastructurally-smooth thick partitioning and volume stitching for larger-scale connectomics

FIB-SEM has become an essential tool for studying neural tissue at resolutions below 10×10×10 nm, producing datasets superior for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 µm thick) optimally...

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Detalles Bibliográficos
Autores principales: Hayworth, Kenneth J., Xu, C. Shan, Lu, Zhiyuan, Knott, Graham W., Fetter, Richard D., Tapia, Juan Carlos, Lichtman, Jeff W., Hess, Harald F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382383/
https://www.ncbi.nlm.nih.gov/pubmed/25686390
http://dx.doi.org/10.1038/nmeth.3292

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382383/
https://www.ncbi.nlm.nih.gov/pubmed/25686390
http://dx.doi.org/10.1038/nmeth.3292

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