Cargando…
Ultrastructurally-smooth thick partitioning and volume stitching for larger-scale connectomics
FIB-SEM has become an essential tool for studying neural tissue at resolutions below 10×10×10 nm, producing datasets superior for automatic connectome tracing. We present a technical advance, ultrathick sectioning, which reliably subdivides embedded tissue samples into chunks (20 µm thick) optimally...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2015
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382383/ https://www.ncbi.nlm.nih.gov/pubmed/25686390 http://dx.doi.org/10.1038/nmeth.3292 |