Cargando…

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA...

Descripción completa

Detalles Bibliográficos
Autores principales: Naito, Yuki, Hino, Kimihiro, Bono, Hidemasa, Ui-Tei, Kumiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382898/
https://www.ncbi.nlm.nih.gov/pubmed/25414360
http://dx.doi.org/10.1093/bioinformatics/btu743